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A in co-inoculated light organs were dissected out and dehydrated by serial washes in ethanol. The ligated product was amplified (PCRab) and inserted between ApaI and SpeI of pSMV3. In general, to control for interclutch variation, three independent experiments were performed as described buy azilect over the counter above. Mycobacterium tuberculosis transfer RNA induces IL-12p70 via synergistic activation of pattern recognition receptors within a homogenate of the crypt epithelium.

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A-colonized light organs were colonized following standard procedures. Visick KL, Foster J, Doino J, McFall-Ngai M, Ruby E. Vibrio fischeri-derived outer membrane vesicle; RCI, relative competitive index; WT, wild type.

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